Fig. 4

Overexpression of Syx7, Ykt6 or Syb attenuates the nephrocyte phenotype induced by APOL1-G1. A Representative images of 10 kD fluorescent dextran particle uptake (red) by nephrocytes from 20-day-old adult flies using nephrocyte-specific driver Dot-Gal4 to overexpress SNARE protein encoding genes Syx7, Ykt6 or Syb, either alone or together with APOL1-G0 or APOL1-G1; carried out at 25 °C. Dotted lines outline individual nephrocytes. B Representative images of LysoTracker (red) uptake by nephrocytes from 20-day-old adult flies using nephrocyte-specific driver Dot-Gal4 to overexpress SNARE protein encoding genes Syx7, Ykt6 or Syb, either alone or together with APOL1-G1; carried out at 25 °C. Hand-GFP transgene expression was visualized as green fluorescence located in nephrocyte nuclei. Dotted lines outline individual nephrocytes. C Quantification of 10 kD dextran uptake in A, relative to wildtype (WT) values. N = 30 nephrocytes total obtained from 6 flies. Results represented as mean ± SD, statistical significance (*) P < 0.05. D Quantification of nephrocyte size in 20-day-old adult flies, relative to WT value. N = 30 nephrocytes total obtained from 6 flies. Results represented as mean ± SD, statistical significance (*) P < 0.05. E Quantification of nephrocyte number in 20-day-old adult flies, relative to wildtype (WT) values. N = 10 flies. Results represented as mean ± SD, statistical significance (*) P < 0.05. (F) Quantification of LysoTracker intensity in B, relative to wildtype (WT) values. N = 30 nephrocytes total obtained from 6 flies. Results represented as mean ± SD, statistical significance (*) P < 0.05