Fig. 3

Knockdown of Syx7, Ykt6 or Syb facilitates the nephrocyte phenotype caused by APOL1-G1. A Mhc-ANF-RFP uptake (red) by nephrocytes from 1-day-old adult flies using the nephrocyte-specific driver Dot-Gal4 to knockdown SNARE protein Syx7, Ykt6 or Syb by RNAi construct, either by itself or together with APOL1-G0 or APOL1-G1; carried out at 25 °C. Dotted lines outline individual nephrocytes. Asterisk indicates a missing nephrocyte. B Representative images of 10 kD fluorescent dextran particle uptake (red) by nephrocytes from larvae using the nephrocyte-specific driver Dot-Gal4 to silence SNARE protein Syx7, Ykt6 or Syb by RNAi construct, either by itself or together with APOL1-G1; carried out at 25 °C. Dotted lines outline individual nephrocytes. C Representative images of LysoTracker (red) by nephrocytes from larvae using nephrocyte-specific driver Dot-Gal4 to silence SNARE protein Syx7, Ykt6 or Syb by RNAi construct, either by itself or together with APOL1-G1; carried out at 25 °C. Dotted lines outline individual nephrocytes. D Quantification of 10 kD dextran uptake in B, relative to wildtype (WT) values. N = 30 nephrocytes total obtained from 6 larvae. Results represented as mean ± SD, statistical significance (*) P < 0.05. E Quantification of LysoTracker intensity in D, relative to wildtype (WT) values. N = 30 nephrocytes total obtained from 6 larvae. Results represented as mean ± SD, statistical significance (*) P < 0.05