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Fig. 6 | Cell & Bioscience

Fig. 6

From: IK is essentially involved in ciliogenesis as an upstream regulator of oral-facial-digital syndrome ciliopathy gene, ofd1

Fig. 6

ofd1 co-injection can rescue ciliary dysmorphology of ik morphants (A) Confocal microscopic images of control MO, ik MO, and ik MO/ofd1 mRNA co-injected Tg(brn3c:GFP) embryos at 2 dpf. Scale bar, 20 µm. Bar graphs show quantification of ciliary length and number in the inner ears of control MO, ik MO, and ik MO/ofd1 mRNA co-injected embryos at 2 dpf. **p < 0.01, ***p < 0.001 (B) Whole-mount immunostaining of pronephric cilia using anti-acetylated-α-tubulin (red) at 2 dpf control MO, ik MO, and ik MO/ ofd1 mRNA co-injected embryos. Scale bar, 20 µm. Stacked bar graph displays the percentage of ciliary morphology of embryos in the anterior pronephric duct (percentage of abnormal ciliary morphology of embryos: control MO, 5.12% (37/39); ik MO, 86.6% (4/30); ik MO + ofd1 mRNA, 16.6% (15/18)

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Cell & Bioscience

ISSN: 2045-3701

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