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Fig. 5 | Cell & Bioscience

Fig. 5

From: IK is essentially involved in ciliogenesis as an upstream regulator of oral-facial-digital syndrome ciliopathy gene, ofd1

Fig. 5

The ciliopathy phenotypes of ik mutants are rescued by ofd1 co-injection (A) The lateral morphological view of control MO, ik MO, and ik MO/ofd1 mRNA co-injected embryos (body curvature and otolith imaged at 2 dpf and pronephric cyst imaged at 3 dpf). Pronephric cyst in ik MO is indicated by red arrowhead. Stacked bar graph displays the phenotypic percentage of embryos. B WISH for ofd1 in control MO, ik MO, and ik MO/ofd1 mRNA co-injected embryos at 1 dpf. Scale bar, 100 μm. Enlarged view of the pronephros in control MO, ik MO, and ik MO/ik mRNA co-injected embryos at 1 dpf. Scale bar, 150 μm. C WISH for foxj1a in control MO, ik MO, and ik MO/ofd1 mRNA co-injected embryos at 2.5 dpf. Scale bar, 100 μm (D) RT-PCR analysis of foxj1a mRNA expression in control MO, ik MO, and ik MO/ofd1 mRNA co-injected embryos at 1.5 dpf. gapdh served as a normalization control. Relative band intensity of foxj1a normalized with respect to gapdh intensity is graphically represented using Image J software (in three individual experiments). *p < 0.05, ***p < 0.001

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Cell & Bioscience

ISSN: 2045-3701

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