Fig. 4

ofd1, a necessary gene for cilia assembly, is downregulated by the loss of ik (A) Heat map of seven differentially expressed cilium assembly genes at least 1.5 > log2 fold changes in ik mutants relative to those of WT embryos. Upregulated DEGs relative to the mean are indicated by red color. Downregulated DEGs are shown by blue color (scale bar, log 2 of mRNA ratio) (B) RT-PCR analysis of ofd1 mRNA expression in WT embryos and ik mutants. gapdh served as a normalization control. Relative band intensity of ofd1 normalized with respect to gapdh intensity is graphically represented (in triplicate) using Image J software. **p < 0.01 (C) WISH for ofd1 in WT embryos and ik mutants at 1 dpf. Scale bar, 100 μm. Enlarged view of the pronephros in WT and ik mutants at 1 dpf. Scale bar, 150 μm (D) RT-PCR analysis of ofd1 mRNA expression in control MO, ik MO, and ik MO/ik mRNA co-injected embryos at 1.5 dpf. gapdh served as a normalization control. Relative band intensity of ofd1 normalized with respect to gapdh intensity is graphically represented (in three individual experiments) using Image J software. **p < 0.01, ***p < 0.001 (E) WISH for ofd1 in control MO, ik MO, and ik MO/ik mRNA co-injected embryos at 1 dpf. Enlarged view of the pronephros in control MO, ik MO, and ik MO/ik mRNA co-injected embryos at 1 dpf. Scale bar, 100 μm