Fig. 3

ik mutants show impaired kidney development with abnormal cilia morphology (A) WISH analysis of pronephric duct markers slc4a4, slc13a1, and slc12a3 in WT embryos and ik mutants at 2 dpf. Scale bar, 250 μm (B) RT-PCR analysis of slc4a4, slc13a1, and slc12a3 expression in WT embryos and ik mutants at 2 dpf. gapdh served as a normalization control. Relative bands intensities of slc4a4, slc13a1, and slc12a3 normalized to gapdh intensity was graphically represented (in triplicates) using Image J software. **p < 0.01, ***p < 0.001 (C) Linear filter model showing correlation of overall gene expression based on RNA-seq data between WT and ik mutants at 3 dpf. The point of foxj1a plot is indicated. (D) RT-PCR analysis of foxj1a expression in WT embryos and ik mutants at 1.5 and 2.5 dpf. gapdh served as a normalization control. Relative band intensity of foxj1a normalized with respect to gapdh intensity is graphically represented (in triplicates) *p < 0.05, **p < 0.01 (E) WISH analysis of foxj1a in WT and ik mutants at 1.5, 2, and 2.5 dpf. Scale bar, 250 μm (F) WISH analysis of foxj1a in control MO, ik MO, and ik MO/ik mRNA co-injected embryos at 2.5 dpf. Scale bar, 250 μm