Fig. 3

In vitro maturation and functional characteristics of iCECs. a Heatmap of RNA expression at different time points in differentiated iCEC. There were 2 independent biological replicates in each sample. b Heatmap of selected gene sets from RNA-Seq at different time points of iCECs differentiation. The unit of the color in each gene is log (FPKM + 1). Blue indicates downregulated transcripts, and red indicates upregulated transcripts. c Volcano plot of differentially expressed genes between day 19 and day 24 of iCECs differentiation. Blue indicates upregulated transcripts on day 19, and red indicates upregulated transcripts on day 24. d GO analysis from RNA-Seq within day 19 versus day 24 iCECs differentiated cells. e qPCR of CECs markers during iCECs differentiation of hESC-derived NCCs. f The potential differences in hESCs, day 19 and day 24 of differentiated iCECs. The image presents the mean value of 10 times per second. g Changes in the potential difference before and after adding 10 µM ouabain to day 24 iCECs. Total time is 120 min (f, g)