Fig. 6
From: Single-cell analysis unveils activation of mast cells in colorectal cancer microenvironment
Spatial co-localization of MCs with fibroblasts and endothelial cells.
a. Spatial plots of H&E staining (column 1), tissue regions (column 2), fibroblasts (COL1A2) (column 3), endothelial cells (PECAM1) (column 4), and MCs (TPSB2) (column 5) in a CRC sample. b. Dot plots display the expression of the regional marker genes (left), MC signature genes (middle), and expression of KIT and KITLG (right) in different tissue regions. c. Immunofluorescence staining of human CRC tissue. KITLG (red), KIT (green), DAPI (blue). Dotted lines demarcate the boundary between the tumor and the stromal regions. d. Spatial visualization of the MC activation signature in the CRC sample. e. Immunofluorescence staining of human CRC tissue and paired NC tissue. TPSAB1 (pink), CMA1 (red), KIT (green), DAPI (blue), in individual and merged channels are shown