Fig. 3
From: Single-cell analysis unveils activation of mast cells in colorectal cancer microenvironment
Heterogeneity of MCs in CRC.
a. UMAP plots of 4,155 MCs colored by cluster (left) and tissue type (center) in the GSE178341 dataset. Bar charts show the proportion of MC subtypes in different tissues (right). b. UMAP plots of MC activated signature. c. Log ratio of average fraction per MC clusters in tumor to normal tissue (top). Mann-Whitney U test, *: p < 0.05, **: p < 0.01, ***: p < 0.001. Dot plots of cytokine and growth factor, protease and histamine, lipid mediator and various receptor-related gene expression in MC clusters (bottom). d. Volcano plot of differentially expressed genes between resting and activated MCs. e. Differential pathway enriched in resting and activated MCs by GSVA, showing the top 10 significant enriched hallmark terms. f. Differentiation trajectory of MCs with each color coded for MC subsets (left), tissue types (center), and pseudotime (right). g. Pseudotime trajectory of CMA1, CPA3, TPSAB1, and KIT expression levels