Fig. 1
From: Single-cell analysis unveils activation of mast cells in colorectal cancer microenvironment
Identification of MC signature genes and decreased MC density in CRC.
a. UMAP plots displaying the major cell types in the GSE164522 (n = 52 samples), GSE178341 (n = 100 samples), and 5-cohorts (n = 189 samples) datasets. b. Dot plots of marker genes for each major cell type in the 5-cohorts dataset. c. Venn diagrams (center) of differential expressed genes (DEGs) in MCs in the GSE164522, GSE178341, and 5-cohorts datasets, with the intersection showing the five MC signature genes (right). The criteria for screening DEGs are depicted within the dotted lines (left). d. Heatmap showing the expression of MC signature genes in each cancer (Normal vs. Tumor). Histogram shows the number of genes with statistical significance (upper). Red represents an increase in tumor expression, green represents a decrease in tumor expression, and only p-values < 0.05 are displayed. e. Heatmap showing the expression of the MC signature genes in 10 bulk RNA-seq cohorts of CRC (Normal vs. Tumor). Blue represents a decrease in tumor expression, dataset source (top), sample size (bottom). f. The expression of TPSAB1 in human CRC tissue and paired NC tissue by Western blotting. g. Immunofluorescence staining of human CRC tissue and paired NC tissue. TPSAB1 (pink), CMA1 (green), CPA3 (green), DAPI (blue), Bar, 200 μm. CRC, colorectal cancer; DEGs, differential expressed genes; FC, fold change; FDR, False Discovery Rate; MC, mast cell; NC, normal colorectum or adjacent colorectum