Fig. 9
Redox imbalance leads to terminal differentiation, aging, and apoptosis of wool follicles in MF wool lambs. a The left: Activity of the PI3K/AKT signaling pathway in longitudinal sections of Meg3 (IG-DMR)-KO mouse skin tissue as determined using immunofluorescence. The letter DP indicates dermal papilla. Middle: detection of the ROS level of 1-d reciprocal cross progeny between ALC and MF using 8-OHDG staining. The black arrow indicates the dermal papilla cell. Right: detection of skin cell apoptosis of 1-d reciprocal cross progeny between ALC and MF using TUNEL staining. b The left: detection of skin cell proliferation of 1-d reciprocal cross progeny between ALC and MF using Ki67 immunostaining. Right: skin section observations of 1-d reciprocal cross progeny by using transmission electron microscopy. The red dotted line indicates the position of dermal papillae, DP: dermal papilla, Nu: cell nucleus, Mito: mitochondria. c Detection of mitochondria DNA copy numbers in the skin tissue of 1-d reciprocal cross progeny between ALC and MF lambs (n = 5; **P < 0.01). d Detection of ATP levels in the skin tissue of 1-d reciprocal cross progeny between ALC and MF lambs (n = 10; ***P < 0.001). e Detection of H2O2 levels in the skin tissue of 1-d reciprocal cross progeny between ALC and MF lambs (n = 10; **P < 0.01). f Detection of reactive oxygen species (ROS) levels in the skin tissue of 1-d reciprocal cross progeny between ALC and MF lambs (n = 10; **P < 0.01)