Fig. 8

Further experiments carried out to verify the candidate signaling pathway using Dlk1-Meg3 (IG-DMR)-knockout (KO) mice model. a Alignment of the genomic DNA sequence of Meg3 (IG-DMR)-wild-type and -KO (− 4675 bp) mice. b Breeding scheme to generate Meg3 (IG-DMR) maternally derived KO mouse heterozygotes by crossing KO heterozygous females with WT males. c The relative expression level of maternally expressed imprinted non-coding RNAs at the Meg3(Gtl2)-miRNAs locus. The primers are shown in Additional file 2: Table S5. d Western blot detection of the difference in PI3K/AKT/mTOR signaling pathway activity between KO and non-KO mice littermates with the same sexes at E14 and P2. e Activity of the PI3K/AKT signaling pathway in longitudinal sections of Meg3 (IG-DMR)-KO mouse skin tissue as determined using immunohistochemical staining. The letter P indicates the primary hair follicle. f Detection of cell apoptosis, proliferation and oxidative stress levels in longitudinal sections of Meg3 (IG-DMR)-KO mouse skin tissue by using Ki67, Caspase 3 and 8-OHDG immunohistochemical staining, respectively. The letter P indicates the primary hair follicle