Fig. 6

Multiple omics analysis revealed the candidate signaling pathway (PI3K/AKT/mTOR/ROS). a The flow chart for multiple omics integrative analysis of miRNA-seq, the proteome, and the metabolome. b Functional classification of downregulated proteins in ALC wool lambskin at P30. c The differentially expressed miRNAs and their potential capacity to target candidate pathways. d Volcano plot of differentially expressed proteins between ALC and MF wool lamb at P30. e Model depicting the role of Gtl2-miRNA locus in inhibiting the PI3K-AKT-mTOR pathway and the subsequent mitochondrial metabolism. The model was drawn using Figuredraw (www.Figuredraw.com). f OPLS-DA scores scatter plot of ALC and MF groups in the positive and negative ion mode (POS and NEG)