Fig. 1

c-Jun inhibits primed to naïve transition. A. Schematic for the derive mouse naive ESC, primed EpiSC and induce naïve ESC differentiation into EpiSC-like cells (naive to primed transition, NPT) under EpiSC culture medium (see methods). And induce primed to naive transition (PNT) (see methods). D indicates the day of the naive to primed transition or primed to naive transition. B. Principal Component Analysis (PCA) of the gene expression (RNA-seq) during naive to primed transition (NPT) and primed to naive transition (PNT). C. Heatmap of naive and primed marker genes expressed during NPT. D. RNA-seq data show the c-Jun expression levels in ESC, a typical naive to primed transition and primed to naive transition time course, EpiSC-like cells, EpiSC and iPSCs. D = day. E. Western blot of the levels of c-Jun protein in ESC, a typical naive to primed transition time course, and EpiSC. D = day. F. Western blot of c-Jun protein in WT ESC, EpiSC-like, c-JunKO ESC and c-JunKO EpiSC-like cells. G. Flow cytometry analysis of the Oct4-GFP (OG2) reporter at primed to naïve transition (PNT) day 3. D = day. Scale bar = 100 μm. H. Effect of knock out of c-Jun in day 3 primed to naive transition. The number of GFP positive colonies was counted on day 3. D = day. Data from 6 biological replicates from 2 independent experiments and shown as the mean ± SEM. *** indicates P value < 0.001, t-test analysis between the c-Jun knock out EpiSClike and WT EpiSC-like cells.