Fig. 6
From: ZSCAN4 interacts with PARP1 to promote DNA repair in mouse embryonic stem cells
The ⍺2 and ZF4 motifs of ZSCAN4 are required for ZSCAN4-associated DSB reduction. A Left: western blot of γH2AX and FLAG (indicative of full length or truncated ZSCAN4) in wildtype mESCs transfected with plasmids expressing either the full length (WT) or truncated (△⍺2ZF4) ZSCAN4. EV: empty vector. Right: quantitative levels of γH2AX. Data are normalized to EV transfected mESCs and are represented as mean ± SEM. B Left: IF images of FLAG and γH2AX in wildtype mESCs transfected with plasmids expressing either the full length (WT) or truncated (△⍺2ZF4) ZSCAN4. Right: quantitative percentage of cells with ≧10 γH2AX foci. Data are represented as mean ± SEM. See also Additional file 1: Table S2