Fig. 5
From: ZSCAN4 interacts with PARP1 to promote DNA repair in mouse embryonic stem cells
The ⍺2 motif on the SCAN domain and the ZF4 motif on the ZF domain are critical for the binding of ZSCAN4 with PARP1. A Illustration of the SCAN domain (top) and its truncated variants (△⍺1 to △⍺5). B Illustration of the ZF domain (top) and its truncated variants (△ZF1 to △ZF4). C Co-IP of the full and truncated FLAG-SCAN domain with the full-length HA-PARP1. D Co-IP of the full and truncated FLAG-ZF domain with the full-length HA-PARP1. E Illustration of the full-length FLAG-ZSCAN4 and two truncated variants (△⍺2ZF2 and △⍺2ZF4). F Co-IP of FLAG-ZSCAN4 (full length), △⍺2ZF2 and △⍺2ZF4 (truncated variants) with the full-length HA-PARP1. G Illustration of the identified interactions between different motifs of ZSCAN4 and PARP1. The arrows indicate inter-domain bindings. The ⍺2 motif on the SCAN domain and the ZF4 motif are labeled to highlight their importance. See also Additional file 1: Table S1