Fig. 2
From: ZSCAN4 interacts with PARP1 to promote DNA repair in mouse embryonic stem cells
ZSCAN4-associated DSB reduction is dependent on PARP1. A Left: western blot of γH2AX and ZSCAN4 in wildtype mESCs transiently overexpressing FLAG-ZSCAN4 treated with or without 3-AB. 0.1% DMSO serves as the vehicle control (no 3-AB). Right: quantitative levels of γH2AX. Data are normalized to no 3-AB (control) group and are represented as mean ± SEM. B IF images of FLAG and γH2AX in wildtype mESCs transiently overexpressing FLAG-ZSCAN4 treated with or without 3-AB. Scale bar: 20 µm. Right: quantitative percentage of cells with ≧10 γH2AX foci. Data are represented as mean ± SEM. C Left: western blot of γH2AX, PARP1 and ZSCAN4 in wildtype (Parp1+/+) and PARP1 knockout (Parp1−/−) mESCs. For each genotype, two clones (#1 and #2) were used. Right: quantitative levels of γH2AX. Data are normalized to clone #1 of Parp1+/+ mESCs and are represented as mean ± SEM. (D) Left: IF images of FLAG and γH2AX in wildtype (Parp1+/+) and PARP1 knockout (Parp1−/−) mESCs. Right: quantitative percentage of cells with ≧10 γH2AX foci. Data are represented as mean ± SEM. See also Figure S2, S3 and Additional file 1: Table S2