Table 3 Detailed components of different differentiation media
Origin | 2013 Huch et al. [41] | 2015 Huch et al. [42] | 2016 Broutier et al. [12] | 2018 Peng et al. [44] | 2018 Hu et al. [45] | 2020 Sorrentino et al. [13] | 2020 Gómez-Mariano et al. [14] | ||
|---|---|---|---|---|---|---|---|---|---|
Mouse | Human | Mouse | Human | Mouse | Human | Mouse | Human | Human | |
Type | Cholangiocyte organoid | Cholangiocyte organoid | Cholangiocyte organoid | Cholangiocyte organoid | Primary hepatocyte organoids | Fetal hepatocyte organoids | Cholangiocyte organoid | Cholangiocyte organoid | Cholangiocyte organoid |
Basal medium | AdDMEM/F12 | AdDMEM/F12 | AdDMEM/F12 | AdDMEM/F12 | William’s E medium | AdDMEM/F12 | AdDMEM/F12 | AdDMEM/F12 | AdDMEM/F12 |
HEPES (mM) | / | / | 10 | 10 | / | √ | / | / | 10 |
GlutaMax | / | / | 1% | 1% | 1% | √ | / | / | 1% |
Antibiotics | / | / | Penicillin–Streptomycin: 1% | Penicillin–Streptomycin: 1% | Penicillin–Streptomycin: 1% Normocin: 0.2% | √ | / | / | Penicillin–Streptomycin: 1% |
Non-essential amino acids | Contained in AdDMEM/F12 | Contained in AdDMEM/F12 | Contained in AdDMEM/F12 | Contained in AdDMEM/F12 | 1% | Contained in AdDMEM/F12 | Contained in AdDMEM/F12 | Contained in AdDMEM/F12 | Contained in AdDMEM/F12 |
B-27 | √ | 1% | 2% | 2% | 2% | √ | √ | √ | 2% |
N2 | √ | 1% | / | 1% | 1% | / | √ | √ | 1% |
N-acetylcysteine (mM) | 1.25 μM | / | 1 | 1 | 1.25 | 1.25 | 1.25 μM | 1.25 μM | 1 |
Rspo1 | / | / | / | / | / | RSPO1 CMa: 15% | / | / | / |
Nicotinamide (mM) | / | / | / | / | 10 | 10 | / | / | / |
Gastrin I (nM) | 10 | 10 | 10 | 10 | / | 10 | 10 | 10 | 10 |
EGF (ng/ml) | 50 | 50 | 50 | 50 | 25 | 50 | 50 | 50 | 50 |
HGF (ng/ml) | / | 25 | / | 25 | 50 | 50 | / | / | / |
FGFs (ng/ml) | FGF10: 100 | FGF19: 100 | FGF10: 100 | FGF19: 100 | / | FGF10: 100 FGF7: 100 | FGF10: 100 | FGF19: 100 | FGF10: 100 |
CHIR99021 (μM) | / | / | / | / | 3 | 3 | / | / | / |
A83-01 (μM) | 0.05 | 0.5 | 0.05 | 0.5 | 1 | 2 | 0.05 | 0.05 | 0.05 |
OSM (ng/ml) | / | / | / | / | / | 10 | / | / | / |
TNFα (ng/ml) | / | / | / | / | 100 | / | / | / | / |
Dexamethasone (μM) | 30b | 30 | 3b | 3 | 3 | 1 | 3b | 3b | / |
DAPT (nM) | 10 | 10 μm | 10 μm | 10 μm | / | / | 10 | 10 | 10 μm |
BMP7 (ng/ml) | / | 25 | / | 25 | / | / | / | 25 | / |