Fig. 2From: Liver organoid culture methodsThe culture and passage procedure for tissue-derived liver organoids. Liver or liver tissues are digested into single cells and then mixed with Matrigel for seeding in plates, and medium is added for culture. For passage, after removing the medium, harvesting solution is added to decompose the Matrigel, and organoid fragments are mixed with fresh Matrigel and seeded. EM expansion mediumBack to article page