Fig. 8

E4f1 regulated spermatogonia mitochondrial function and fatty acid metabolism. A Transmission electron microscopy images of spermatogonia from PD6 control and E4f1 cKO testes. B Quantification of mitochondrial length in spermatogonia from PD6 control and E4f1 cKO testes. C Quantification by FACS of the mean JC1 red/green fluorescence ratio values of THY1⁺ cells from PD6 control and E4f1 cKO testes. D Quantification by FACS of the mean fluorescence intensity of MitoSOX™ red in THY1⁺ cells from PD6 control and E4f1 cKO testes. E Relative intensity of metabolites involved with pyruvic acid in THY1⁺ cells from PD6-8 control and E4f1 cKO testes. F Relative intensity of several fatty acids in THY1⁺ cells from PD6-8 control and E4f1 cKO testes. G Fold enrichment of DNA obtained with ChIP analysis with E4F1 versus normal mouse IgG by real-time PCR. n >  = 3. Error bars represent SEM. *p < 0.05, Student’s t test. H Immunofluorescence staining for gH2AX (red) and UCHL1 (green) in sections of PD14 control and E4f1 cKO testes. I Percentage of gH2AX-positive undifferentiated spermatogonia in testis sections from PD14 control and E4f1 cKO testes. n = 3