Fig. 6

scRNA-seq defines the transcriptomes of E4f1 cKO spermatogonia. A The resulting UMAP plots show unbiased clustering of germ cells extracted from testicular cells of controls and E4f1 cKO mice and the resulting UMAP plots of integrated germ cells of control and E4f1 cKO testes. B Integrated germ cell trajectories with cells ordered in pseudotime, states, and origin by unbiased dynamic lineage analysis. C Expression levels (vertical axis) of marker genes during spermatogonia development among integrated germ cells ordered in pseudotime (cell coloring id according to states from (B)). D Heatmap of marker gene expression patterns corresponding to the pseudotime trajectory of integrated germ cells. E Dotplot for the expression of selected marker genes across germ cell types in controls and E4f1 cKO mice. F Summary schematic depicting the percentage of spermatogonia in each cellular state in controls and E4f1 cKO mice