Fig. 4

Genetic inactivation of E4f1 blocked spermatogonia development. A Whole mounts of seminiferous tubules from PD14 control and E4f1 cKO mice stained with LIN28. B The percentage of A_s, A_pr, and A_al spermatogonia. C Immunofluorescence staining for EdU (green) and LIN28 (red) in sections of PD14 control and E4f1 cKO testes. D The percentage of EdU⁺ LIN28⁺ cells in A_s, A_pr, and A_al spermatogonia. E Immunofluorescence staining for c-Kit (red) and SOX9 (green) in sections of PD6 and PD14 control and E4f1 cKO testes. F Quantification of c-Kit⁺ spermatogonia per 500 Sertoli cells in testis sections from PD6 and PD14 control and E4f1 cKO mice. n = 3. G Immunofluorescence staining for KIT (green) and FOXO1 (red) in sections of PD6 control and E4f1 cKO testes. H Quantification of Kit⁺ spermatogonia of FOXO1⁺ germ cells in testis sections from PD6 control and E4f1 cKO mice. n = 3. Error bars represent SD. *P < 0.05, **P < 0.01, ***P < 0.001, Student’s t test