Table 1 Antibodies applied

Primary antibodies

Anti-mouse Tau (Clone: RTM47)

Rat, monoclonal

FUJIFILM Wako, 012–26963

1:1000 (IHC), 1:3000 (WB)

Anti-human Tau (Clone: RTM49)

Rat, monoclonal

FUJIFILM Wako, 015–26953

1:1000 (IHC)

Anti-Tau (316–355 aa, clone: 77G7)

Mouse, monoclonal

Biolegend, 816701

0.4 µg/ml (WB), (CDI, CSA)

Anti-Human PHF-Tau (clone: AT8)

Mouse, monoclonal

ThermoFisher, MN1020

1:400 (IHC), 1:1000 (WB)

Anti-Tau, x-421 (TauC3)

Mouse, monoclonal

BioLegend, 806304

1:400 (IHC)

Anti-3R Tau

Rat, monoclonal

FUJIFILM Wako, 016–26581

1:4000 (WB)

Anti-RD3 Tau (clone: 8E6/C11)

Mouse, monoclonal

Gift from Dr. R. de Silva

(CDI)

MAP2

Rabbit, polyclonal

Synaptic systems, 188002

1:1000 (IHC)

Homer 1/2/3

Rabbit, polyclonal

Synaptic systems, 160103

1:500 (IHC), 1:2000 (WB)

Bassoon

Chicken, polyclonal

Synaptic systems, 141016

1:500 (IHC)

Bassoon

Rabbit, monoclonal

Cell signaling, D63B6

1:3200 (WB)

Synaptophysin 1/2

Mouse, monoclonal

Synaptic systems, 101111

1:2000 (WB)

PSD95

Rabbit, polyclonal

Invitrogen, PA5-85783

1:2000 (WB)

GAPDH (HRP Conjugate, 14C10)

Rabbit, monoclonal

Cell signaling, 50-190-709

1:20,000 (WB)

Secondary Antibodies

Anti-Rabbit IgG (H + L)/Alexa Flour 488

Goat, polyclonal

Invitrogen, A11008

1:500 (IHC)

Anti-Rabbit IgG (H + L) / Alexa Flour 633

Goat, polyclonal

Invitrogen, A21070

1:500 (IHC)

Anti-Rat IgG (H + L)/Alexa Flour 633

Goat, polyclonal

Invitrogen, A21094

1:500 (IHC)

Anti-Chicken IgY (H + L)/Alexa Fluor 633

Goat, polyclonal

Invitrogen, A21103

1:500 (IHC)

HRP anti-Rat IgG (clone: Poly4054)

Goat, polyclonal

BioLegend, 405405

1:3000 (WB)

Anti-mouse IgG/HRP

Sheep, polyclonal

Amersham, NA931-1ML

1:3000 (WB)

Anti-rabbit IgG/HRP

Donkey, polyclonal

Amersham, NA934-1ML

1:3000 (WB)