Fig. 5
From: Neuronal MHC-I complex is destabilized by amyloid-β and its implications in Alzheimer’s disease
Aβ-resistance of MHC-I increased the interaction of NCAM1–MHC-I. A Schematic representation of the structure of the single-chain trimer (SCT). The SCT construct was composed of three genes (MHC-I, β2M, and antigen peptide), which resulted in the expression of a single protein composed of these three proteins. B–D Immunoblot analyses of endogenous MHC-I and exogenous SCT levels in oAβ-treated SH-SY5Y cells. Biotinylated surface MHC-I and SCT were immunoprecipitated with streptavidin and analyzed by immunoblotting (B, n = 4). The levels of total (C) and surface (D) MHC-I and SCT were normalized to the levels of actin. The data were analyzed by unpaired two-tailed Student’s t-test (C, D). E–H Coimmunoprecipitation analysis of exogenous HLA-A2.1–NCAM1-HA (E, F; n = 4) and SCT–NCAM1-HA (G, H; n = 4) interactions in oAβ-treated SH-SY5Y cells. The levels of coimmunoprecipitated NCAM1-HA (F, H), HLA-A2.1 (F), and SCT (H) were normalized to actin levels, and the data were analyzed by unpaired two-tailed Student’s t-test (F, H). The data are presented as the mean ± SEM (N.S., not significant; *P < 0.05, **P < 0.001, ***P < 0.005)