Fig. 2

Pathologic changes of neuronal MHC-I–β₂M complex in the brains of AD patients. A–C Immunoblot analyses of MHC-I, β₂M, PSD95, synaptophysin, VGAT, and NeuN in subcellular fractions from aged normal and AD brains. The levels of MHC-I (B) and β₂M (C) in synaptosomes were normalized to total protein levels in all fractions (n = 6). D–F Immunoprecipitation analyses with specific antibodies of MHC-I-β₂M complex and β₂M-free MHC-I in synaptosomes purified from aged normal and AD brains (C: n = 6). The levels of the purified β₂M interacting (E) and β₂M-free (F) forms of MHC-I in synaptosomes were normalized to total protein levels. Data are presented as the mean ± standard error of the mean (SEM) (N.S., not significant; *P < 0.05, **P < 0.001 by unpaired two-tailed Student’s t-test)