Fig. 3

Mettl1 modulates the transcriptome landscape of internal m⁷G-modified mRNA during neurogenesis. (A, B) RNA dot blot analysis of m⁷G levels in NSCs, astrocytes and neurons. n = 3, *P < 0.05 compared with NSCs (100 ng); ##P < 0.01 compared with NSCs (200 ng). (C, D) RNA dot blot analysis of m⁷G levels in NSCs treated with Mettl1 shRNAs. n = 3, ***P < 0.001 compared with NSCs (100 ng); ###P < 0.001 compared with NSCs (200 ng). (E, F) RNA dot blot analysis of m⁷G levels in NSCs treated with Mettl1 overexpression. n = 3, ***P < 0.001 compared with NSCs (100 ng); ##P < 0.01 compared with NSCs (200 ng). (G) Distribution of internal m⁷G across NSCs, neurons and astrocytes mRNA segments. (H) Pie charts presenting the fraction of m⁷G peaks in NSCs, neurons and astrocytes. (I) Motif analysis of internal mRNA m⁷G in NSCs, neurons and astrocytes mRNAs (J, K) Bar plot chart showing the significant GO terms and KEGG analysis for NSCs and differentiated neurons mRNAs containing internal m⁷G. (L) Volcano plot of significantly altered internal mRNA m⁷G peaks in NSCs compared to neurons. NSCs, neural stem cells; Neu, neurons; Ast, astrocytes. Data are represented as the mean ± SEM. NSCs, neural stem cells; Sh-Mettl1, shRNA Mettl1; OE-Mettl1, overexpressing Mettl1; n represents number of independent experiments