Fig. 2

Mettl1 promotes neuronal differentiation of NSCs.. (A,B) Western blotting and RT-qPCR of Mettl1 expression in NSCs treated with Mettl1 shRNAs. n = 3, ***P < 0.001, **P < 0.01 compared with control. (C-F) Immunofluorescence staining and quantification for Tuj1, GFAP and EdU on NSCs treated with control or Mettl1 shRNA. Scale bar, 200 μm. n = 3, ***P < 0.001, **P < 0.01 compared with control. (G,H) Western blotting and RT-qPCR of Mettl overexpression in NSCs. n = 3, ***P < 0.001 compared with control. (I-L) Immunofluorescence staining and quantification for Tuj1, GFAP and EdU on NSCs treated with control or Mettl1 overexpression. Scale bar, 200 μm. n = 3, ***P < 0.001, **P < 0.01 compared with control. Data are represented as the mean ± SEM. NSCs, neural stem cells; Sh-Mettl1, shRNA Mettl1; OE-Mettl1, overexpressing Mettl1; n represents number of independent experiments