Fig. 4
From: NADH elevation during chronic hypoxia leads to VHL-mediated HIF-1α degradation via SIRT1 inhibition
HIF-1α acetylation is increased during chronic hypoxia. (A ~ D) HIF-1α acetylation was determined in immunoprecipitated HIF-1α preparations, followed by probing with anti-acetyl-K antibody, in HeLa cells exposed to hypoxia for 9 and 24 h, under condition of MG132 treatment. HIF-1α immunoprecipitates were prepared with anti-HIF-1α or anti-Flag-antibodies from the cells accumulating HIF-1α endogenously (A) or exogenously through transfection with Flag-tagged HIF-1α (B and C). The effect of chronic hypoxia condition (A), pyruvate (B), and Myc-tagged SIRT1 transfection (C) on the acetylation was monitored. (D) Pyruvate/NADH-sensitive recovery in HIF-1α acetylation. HIF-1α acetylation was determined in the presence of pyruvate or NADH under chronic hypoxia condition