Fig. 6

Tau PFF alters the trans-Golgi network in an integrin-dependent manner. (A) Representative immunoblotting and Coomassie staining of protein secretion in primary astrocytes exposed to tau-PFF together with the indicated inhibitors. n = 3 biological repeats. (B) The mRNA expression of the indicated genes from the RNAseq study in Fig. 3. Error bars, means ± SD. ***, p < 0.001, ****, p < 0.0001 by one-way ANOVA. n = 3 biological repeats. (C) Tau PFF treatment alters the trans-Golgi network (TGN) in PAs. Primary astrocytes treated with either PBS or tau PFF for 6 h were stained with antibodies against the following organelle markers, Calreticulin (ER), ERGIC53 (ERGIC), GM130 (cis-Golgi), VTI1B (TGN) together with a DNA dye (blue). Scale bard, 10 μm. (D) Tau PFF-induced Golgi fragmentation is inhibited by PF562271 (PF271). Cells treated with PBS, tau PFF alone or in combination with PF271 or PF271 alone were stained by VTI1B antibodies (green) and DAPI (blue). Scale bar, 10 μm. Images represent 3 biological repeats