Fig. 6

Drosophila behavior patterns. A Drosophila behavior feature correlation network. B, PCA plot for drosophila behavior of different strains. C–D Move time proportion (C), acquaintance (D) of drosophila showed a different changing pattern over the videotaped timeline. E–L Speed (E), move time (F), angular velocity (G), track straightness (H), acquaintance (I), interaction episode count (J), social space distance (L) changing patterns of flies in the chamber. Statistical analysis: Welch Two Sample t-test was used for comparisons between two groups. *P < 0.05, **P < 0.01, ***P < 0.001. Non-significant test was not annotated in the graph for brevity. n = 27 videos for male w¹¹¹⁸ flies, n = 27 videos for female w¹¹¹⁸ flies, n = 26 videos for male OR flies, n = 27 videos for female OR flies, n = 27 videos for male CS flies, n = 27 videos for female CS flies were recorded and analyzed. All videos recorded were from three independent experiment replicates. Multi-level correlation of paired behavior metrics was calculated to build the network. The network plot drawn metric relationships with magnitude of correlation larger than 0.7 and FDR q-value < 0.05. The edge width is proportional to correlation magnitude. And a red edge is sign of positive correlation and green edge is for negative correlations. The node color represents cluster identification of metric made by kmeans cluster algorithm