Fig. 5

Circadian rhythm in fly behavior. A–D, move length (A), move time (B), acquaintances (C), interaction episode count (D) showed a circadian rhythm from ZT0 to ZT11. E Difference of total move length emerged at about 10 min after recording start. F Difference of move time emerged at about 10 min after recording start. G, H Avg. number of crowded dro. G and social space distance (H) did not follow circadian rhythm. Statistical analysis: n = 6 videos for male w¹¹¹⁸ flies for each hour from ZT0 to ZT11 (A–D, G, H). For detailed comparison of behavior at ZT01, ZT06 and ZT11(E, F), n = 6 videos for male w¹¹¹⁸ flies at ZT01, n = 6 videos for female w¹¹¹⁸ flies at ZT01, n = 6 videos for male w¹¹¹⁸ flies at ZT06, n = 6 videos for female w¹¹¹⁸ flies at ZT06, n = 6 videos for male w¹¹¹⁸ flies at ZT11, n = 6 videos for female w¹¹¹⁸ flies at ZT11 were recorded and analyzed