Fig. 2
Biased and crowded drosophila social interaction. A representative drosophila social network. The right-up panel showed interactions with 4th fly occupied a larger proportion in 1st fly’s interaction pie. B Comparison of acquaintance of simulated random social network with that of male w1118 flies under normal condition. C Acquaintance was higher for flies in red dim darkness compared with that in normal lighting. D Acquaintance was higher for flies after 6 days sleep deprivation. E w1118; +;TH/+; flies had higher acquaintance than w1118 flies. F Crowded drosophila in an interaction/encounter event. G Comparison of avg. number of crowded dro. of simulated random social network with that of male w1118 flies under normal condition. H Red dim darkness lowered avg. number of crowded dro. I Avg. number of crowded dro. did not change after 6 days sleep deprivation. J no significant changes were found for w1118; + ;TH/ + ; male flies compared with w1118 male flies. Statistical analysis: Welch Two Sample t-test was used for comparisons between two groups. *P < 0.05, **P < 0.01, ***P < 0.001. Non-significant test was not annotated in the graph for brevity. For subfigure B, G, 27 videos for male w1118 flies under normal condition were recorded and analyzed. For subfigure C and H, 27 videos for male w1118 flies in darkness and 27 videos for male w1118 flies under normal lighting were recorded and analyzed. For subfigure D and I, 23 videos for male w1118 flies after 6 days sleep deprivation and 24 videos for age matched male w1118 flies without sleep deprivation were recorded and analyzed. For subfigure E and J, 24 videos for male w1118; + ;TH/ + ; flies and 24 videos for age matched male w1118 flies were recorded and analyzed. All videos were from three independent experiment replicates. The color-coded track in 2C indicates the fly traces in last 15 video frames (0.5 s). The average-by-video value of features were plotted in subfigure B–E, G–J, similarly hereinafter. We annotated newly proposed metrics with the superscript Ξ/ksi/ for subfigures B–J, similarly hereinafter