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Table 1 Composition and nutrient levels of diets used in this study

From: Modelling porcine NAFLD by deletion of leptin and defining the role of AMPK in hepatic fibrosis

Growth stage

Feed

Feed stuff

Guaranteed value of component analysis

Feed additives

Nutrition content of pig diets

Weaning-45 Days

AnYou-810 formula feed for suckling pigs (Anyou Feed Co. LTD, China)

Bean pulp, corn particle, Flour. soybean oil, sodium chloride, choline chloride, copper sulfate, zinc chloride, manganese sulfate, L -lysine hydrochloride, DL-methionine, Vitamin A, E, D3, phytase

Crude protein (%) ≥ 19.5, crude fat (%) ≥ 2.8, crude ash (%) ≤ 8.0, crude fiber ≤ 4.0, calcium (%) 0.5–1.1, total phosphorus (%) ≥ 0.3, sodium chloride (%) 0.3–0.9. lysine (%) ≥ 1.6. water (%) ≤ 14.0

Aureomycin premix 75 mg/kg, Kitasamycin premix 50 mg/kg, Bacitritin zinc premix 40 mg/kg

45–85 Days

MuWang-352 formula feed for piglets (MuWang Feed Co. LTD China)

Corn, soybean meal, soybean oil, calcium hydrogen phosphate, calcium carbonate, sodium chloride, lysine, vitamin A, D3, E, K3, B12, copper sulfate, zinc sulfate. manganese sulfate, ferrous sulfate, sodium selenite, calcium iodate

Crude protein (%) ≥ 17.5, crude ash (%) ≤ 7.0, crude fiber ≤ 5.0, calcium (%) 0.5–1.0. total phosphorus (%) ≥ 0.5. sodium chloride (%) 0.3–0.8, lysine (%) ≥ 1.0, water (%) ≤ 14.0

Aureomycin premix 72 mg/kg, Flavamycin premix 24 mg/kg

85 Days

Purina-832980 formula feed for boar (Agribrands Purina Feedmill

(Langfang) Co., Ltd, USA & China)

Corn, soybean meal, bran, calcium hydrogen phosphate, calcium carbonate, sodium chloride, L-lysine, vitamins A, D3, E, B2, B12, copper sulfate, ferrous sulfate, zinc sulfate, manganese sulfate, sodium nitrite, phytase, etc

Crude protein (%) ≥ 13, crude ash (%) ≤ 10.0, crude fiber ≤ 10.0, calcium (%) 0.4–1.5, total phosphorus (%) ≥ 0.3. sodium chloride (%) 0.3–0.1.5, lysine (%) ≥ 0.45, water (%) ≤ 14.0

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Sow pregnancy

MuWang-356

formula feed for piglets (MuWang Feed Co. LTD, China)

Corn, soybean meal, soybean oil, calcium hydrogen phosphate, calcium carbonate, sodium chloride, lysine, vitamin A, D3, E, K3, B12, copper sulfate, zinc sulfate, manganese sulfate, ferrous sulfate, sodium selenite, calcium iodate

Crude protein (%) ≥ 14.0. crude ash (%) ≤ 8.0, crude fiber ≤ 7.0, calcium (%) 0.6–0.95, total phosphorus (%) ≥ 0.5. sodium chloride (%) 0.3–0.8, lysine (%) 0.65, water (%) ≤ 14.0

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Lactation period

MuWang-357 formula feed for piglets (MuWang Feed Co. LTD, China)

Corn, soybean meal, soybean oil. calcium hydrogen phosphate. calcium carbonate, sodium chloride, lysine, vitamin A, D3, E, K3, B12, copper sulfate, zinc sulfate manganese sulfate, ferrous sulfate, sodium selenite, calcium lodate

Crude protein (%) ≥ 16.0. crude ash (%) ≤ 8.0. crude fiber ≤ 7.0. calcium (%) 0.6–0.95, total phosphorus (%) ≥ 0.5, sodium chloride (%) 0.3–0.8, lysine (%) 0.8, water (%) ≤ 14.0

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Nutrition content of rat diets

Weaning-growth

HuaFukang-1022 Rat growth maintenance feed (Beijing Hua Fu Kang Biotechnology Co. LTD, China)

Soybean meal, fish meal, Vegetable oil, bran, corn, Vitamins A, D, E, B1, B12, B6, Pantothenic acid, calcium bicarbonate, Calcium carbonate, copper sulfate, zinc sulfate, manganese sulfate, ferrous sulfate

Crude protein (%) ≥ 18.0, crude fat (%) ≥ 4.0, crude ash (%) ≤ 8.0, crude fiber ≤ 5.0, calcium (%) 1.0–1.8, total phosphorus (%) 0.6–1.2, water (%) ≤ 10.0

  1. In this table, the composition and nutrient levels of each diets used in the study for pig and rat are listed in details