Fig. 3
From: Modelling porcine NAFLD by deletion of leptin and defining the role of AMPK in hepatic fibrosis
Pathological and molecular detection of liver injury in LEPTIN−/− pigs. Triglyceride concentrations (A) and expression of genes related to FFA synthesis (B) in LEPTIN mutant and WT pigs. Expression of inflammation related genes in liver (C) and the concentration of inflammatory cytokines, IL-1β, IL-6 and TNF-α in serum (D). N = 3–5/group. E Gross images, H&E and immunofluorescent staining for α-SMA in 25-month-old LEPTIN mutant and WT livers (bar = 20 μm). F Liver functional status and expression of liver fibrosis markers in serum. G Expression of genes related to fibrogenesis. N = 3–5/group. The bars represent the mean ± SD; *P < 0.05; NS non-significant