Fig. 3

Specific markers of resident LPCs- and parenchymal cells-derived LPCs by transcriptomic profiling. a Venn diagrams showing numbers and overlap of genes upregulated in three cultured LPCs. b Ten upregulated gene ontologies (biological process) in three cultured LPCs. c Protein–protein interactions of DEG products in resident LPCs-derived LPCs related to gene ontologies b were constructed using the STRING database. The size and color of the circles depict the average absolute expression level. d Normalized gene counts of the 5 most significantly differentially expressed genes from RNA-sequencing a and validation by RT-q-PCR. Gene expression levels were normalized to Actin level and data were plotted as mean ± SD of three independent experiments. e Heat map showing expression levels of specific markers in three cultured LPCs, DDC-hepPD, and CCl₄-CD24 + LPCs. f Normalized expression levels of 5 most significantly differentially expressed genes of resident LPCs-derived LPCs in fibrotic mice after CCl₄ injections. g Venn diagrams showing numbers and overlap of genes uniquely upregulated in resident LPCs-derived LPCs by comparison with the NASH mice fed with FFC diet. h Normalized gene counts of 5 most significantly differentially expressed genes of resident LPCs-derived LPCs in RNA-sequencing g and their validation by RT-q-PCR. Gene expression levels were normalized to Actin and data are plotted as mean ± SD of three independent experiments. Resident LPCs-derived LPCs, reLPCs; Hepatocytes-derived LPCs, HepLPCs; BECs-derived LPCs, BecLPCs. For panels d, f, and h, data summarize 3 independent experiments. *p < 0.05; **p < 0.005; ***p < 0.001; ns: no significant difference.