Fig. 5

Gonad hormones in F1 females mask lipid phenotypes of paternal iAs. (a) Liver triglyceride (TG) contents in F1 females in the fasting condition after sham or OVX, n = 5–6 mice. (b) H&E staining of the liver in F1 females after sham or OVX. Scale bar, 100 μm. (c-d) Serum TG and non-esterified fatty acids (NEFAs) levels in F1 females after sham or OVX, n = 5–6 mice. (e-g) RT-qPCR analysis of genes in lipogenesis, fatty acid oxidation (FAO), and lipolysis in the liver of F1 females in the fasting condition after sham or OVX, n = 5–6 mice. The mean value in the con-sham group was set as 1. Two-way ANOVA with the Holm-Sidak method was used to analyze liver TG, serum TG, serum NEFAs, and gene expression levels. Data are mean ± S.E.M. * P < 0.05 between conF1 and iAsF1 groups under the same conditions