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Fig. 4 | Cell & Bioscience

Fig. 4

From: Menin orchestrates hepatic glucose and fatty acid uptake via deploying the cellular translocation of SIRT1 and PPARγ

Fig. 4

Menin binds to the promoters of fatty acid binding protein genes as the transcriptional targets of Menin in mouse hepatocytes. A. Several amplicons (P1, P2, etc.) were designed to detect the indicated promoter regions of the Fabp3, Fabp4, and/or Fabp5 genes for Menin-ChIP assays. TSS represents the transcription start site, and curved arrows represent their transcription directions. B. Results of quantitative Menin-ChIP PCR assays in plain mouse hepatocytes from three independent Menin-ChIP experiments, showing the percentage of input chromatin bound by Menin, indicated that Menin occupied the promoter regions of downstream target genes of the PPAR signaling pathway, Fabp3, Fabp4, and/or Fabp5. *P < 0.05, **P < 0.01. C. Representative ChIP-PCR results of promoter regions of Fabp3 (upper panel), Fabp4 (middle panel), and Fabp5 (lower panel) from anti-Menin ChIPs in Menin over-expression (mMen1) and Menin-knockdown (siMen1) hepatocytes, as well as their corresponding controls (Vector and Control)

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Cell & Bioscience

ISSN: 2045-3701

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