Fig. 5
From: A novel 3D bilayer hydrogel tri-culture system for studying functional motor units
Ca2+ imaging a Representative field of a tri-culture in bright field illumination (top left and right), Fura-2 fluorescence (380 nm; bottom left), and mApple fluorescence (605 nm; bottom right). Red arrows indicate a motor neuron and blue arrows indicate myotubes. b Representative trace shows a typical response observed in MNs during stimulation with 50 mM K + . c Bar graph displays MN baseline and depolarization-evoked 50 mM K +) peak amplitude [Ca2 +]i (n = 8). P = 0.008. Wilcoxon matched-pairs signed rank test. d Representative trace shows a typical myofiber response to ACh (10 μM) stimulation. e Bar graph displays myotube baseline and ACh (10 μM) evoked peak amplitude [Ca2 +]i (n = 8), * p = 0.02 paired Student’s t-test. Data presented are means ± SD and * p < 0.05, ** p < 0.01.