Fig. 2

Matrigel-Schwann cell layer contributes to MN differentiation and myelination a Experimental scheme for determining the effects of the Matrigel layer. On day 6 of the diagram in Fig. 1b, Schwann cells were mixed with (+) Matrigel group, but not in without (−) Matrigel group. The motor neuron differentiation and myelination were determined on day 14. b Immunostaining showed the differentiated motor neurons in (+) Matrigel group. The morphology of motor neurons was observed by neural marker Tuj1 (red), and all the nuclei were detected by DAPI (blue). c Quantification data comparing axon length in (+) Matrigel group (n = 6) and without (−) Matrigel group (n = 6). d and e Immunostaining showing the myelination of an axon and axon terminal with (+) Matrigel (d) and without (-) Matrigel (e). Myelination was detected using myelin-basic protein (MBP, green). Results were presented as means ± SD, ***p ≤ 0.001, compared with (+) Matrigel group.