Fig. 8

GLUT1 and GOT2 expression in pregnancies complicated by intrauterine growth restriction. Western Blot of GOT2 level in term placental explants exposed either to low or high FSS (a) and band densitometry (b). GOT2 intensity in IUGR placentas (c) and the representative staining for data shown in (f). Western blot (d) and qPCR (e) of placental GOT2 levels in IUGR and control cases. Analyses were performed with a minimum of five placenta samples for Western Blot, nine IUGR placenta samples for qPCR, and 20 different IUGR placenta for IF. Immunofluorescence picture represents GOT2 (red) and GLUT1 (yellow). Nuclei are stained with DAPI (f). GLUT1 expression is not significantly affected in IUGR placenta compared to control in all analysis (g and h). Key enzymes of the glycolytic pathway HK2 (i) and PFKP (j) are slightly upregulated in IUGR compared to CTRL placentas. Outlier were detected with Grubbs’ α = 0.05, normal distribution of data was analyzed with Shapiro–Wilk test. Statistical analysis was performed using one sample t-test for the protein analysis. Statistical analysis for the immunofluorescent staining was performed with an unpaired t-test. Statistical significance was set at p < 0.05. Values represent mean ± SEM