Fig. 6
From: Fluid shear stress induces a shift from glycolytic to amino acid pathway in human trophoblasts
Dependence of GOT1 and GOT2 from glucose availability Efficiency of SLC2A1 silencing in differentiated BeWo cells was determined for CTRL and silenced samples on mRNA (a) and protein level (b and c). Western Blot (d) and band densitometry (e) revealed increased GOT1 protein levels, while GOT2 levels (f and g) remained unchanged in SLC2A1-silenced cells. Cells were cultured with three different glucose concentrations. Immunoblotting (h) and band densitometry (i) showed declining GLUT1 levels with decreasing available glucose concentrations. GOT1 (j and k) and GOT2 (l and m) levels increased when cultured in low glucose medium. Experiments were performed a minimum of three times. Outlier were detected with Grubbs’ α = 0.05, normal distribution of data was analyzed with Shapiro–Wilk test. Statistical analysis was performed using one sample t-test for the protein analysis. Statistical significance was set at p < 0.05