Fig. 4

Oncogenic SIRT7 depends on CHD1L to augment RCC malignant aggressiveness. A Boxplot showing the differential expression levels of SIRT7 in normal and tumor samples from TCGA-KIRC. B Kaplan–Meier survival curves comparing the differential prognosis of SIRT7-high or -low KIRC patients. C CCK-8 assays show the growth rates in cells transfected with EV, wild-type SIRT7, or H187Y plasmids. D Western blotting assays reveal the protein levels of SIRT7 in control or SIRT7-KD cells. E CCK-8 assays show the growth rates in SIRT7-KD cells with or without CHDL1 restoration. F Colony formation assays show colony formation abilities in SIRT7-KD cells with or without CHDL1 restoration. G–I Colony formation, migration, or sphere formation assays were conducted in the indicated groups. The representative images and quantification data were shown in each assay. J–L SIRT7 overexpression enhanced the xenograft tumor growth of 786-O cells, and CHD1L KD could abolish this effect. The representative tumor image in the indicated groups is shown on the left (J). The tumor volumes were generated (K), and tumors were weighed (L). *p < 0.05, **p < 0.01, ***p < 0.001, ns no significant