Fig. 1

High CHD1L correlated with poorer prognosis of RCC patients. A siRNA KD of 9 candidate CHD family members and their effects on the growth of RCC (786-O or Caki-1) cells. The quantitative results shown are representative of 5 experiments. B CCK-8 assays revealed the growth rates of RCC cells transfected with shCtrl or shCHD1L#1/2 lentiviruses, individually. C Representative immunohistochemistry (IHC) staining graphs of CHD1L in normal or RCC samples with different tumor grades. D The distribution of the difference in CHD1L immunoreactivity score (△scores = (Tumor − Normal)/Normal). The CHD1L staining scores were quantified in 120 pairs of tumors from the Ruijin-RCC dataset. E Correlation analysis was conducted via the Kruskal–Wallis test to confirm the relationships between CHD1L h-scores and hazard clinical characteristics. F, G Kaplan–Meier survival curve analysis was conducted to compare the prognostic differences between CHD1L-high and CHD1L-low patients in Ruijin-RCC or TCGA-KIRC datasets. *p < 0.05, **p < 0.01, ***p < 0.001, ns no significant