Fig. 3
From: Expression of expanded GGC repeats within NOTCH2NLC causes cardiac dysfunction in mouse models
Generation of Myh6-tg mice that express mutant polyG in cardiomyocytes. A Schematic diagram of generation of the Myh6-tg mice. B Representative immunoblot for polyG in the heart of wild-type and Myh6-tg. Vinculin was used as a loading control. C Immunoblotting analysis of polyG in the oil or tamoxifen injected heart, gastrocnemius, liver, and cortex. Vinculin was used as a loading control. D Immunofluorescent staining against Flag in the hearts of control and Myh6-tg mice at P90. Red, flag (polyG); cyan, DAPI. Scale bar, 50 μm. E Co-immunofluorescent staining against Flag and p62 in the hearts of control and Myh6-tg mice at P90. Red, p62; Green, flag (polyG); cyan, DAPI. Scale bar, 10 μm. F Colocalization of p62 with polyG inclusions in the heart of Myh6-tg mice. N = 3 mice per group, three images per mouse were used to count