Fig. 2

PolyG proteins impair ventricular structure in the EIIa-tg mice. A Representative 2-chamber cross-section, Hematoxylin-eosin stained at P30. Scale bar, 500 μm. B Hematoxylin–eosin staining showed mild infiltrating cells in the transgenic mice at P30. Scale bar, 40 μm. C Representative images from Masson-stained transgenic mice and control mice at P30. Scale bar, 40 μm. D Quantification of myocardial fibrotic area. **P < 0.01. E Representative M-mode echocardiography of the LV chamber. F–J Assessment of LVID at end systole and diastole (F), LVV at end systole and diastole (G), EF (%) (H), FS (%) (I), and LVPW thickness at end systole and diastole (J). n = 6 mice per group for all experiments. Data are presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, Student’s t test. LV left ventricular, LVID left ventricular internal dimension, LVV left ventricular volume, EF ejection fraction, FS fractional shortening, LVPW left ventricular posterior wall