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Fig. 2 | Cell & Bioscience

Fig. 2

From: Gut microbial metabolite deoxycholic acid facilitates Th17 differentiation through modulating cholesterol biosynthesis and participates in high-fat diet-associated colonic inflammation

Fig. 2

The effect of DCA on Th1 and Th2 cell differentiation. a Splenocytes were differentiated under Th0 or Th1-polarizing conditions for 72 h in the absence or presence of different dosage of DCA, then the cells were re-stimulated with PMA/ionomycin and analyzed for the percentages of IFN γ-producing CD4+ T cells by flow cytometry (n = 3). b Splenocytes were differentiated under Th0 or Th2-polarizing conditions for 72 h in the absence or presence of different dosage of DCA, then the cells were re-stimulated with PMA/ionomycin and analyzed for the percentages of IL-4-producing CD4+ T cells by flow cytometry (n = 3). n.s.: no statistically significant difference (p > 0.05). One-way ANOVA with Tukey's multiple comparisons tests were used (a and b right panel). Data are expressed as mean ± SEM from at least three independent experiments or representative data (a and b left panel)

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Cell & Bioscience

ISSN: 2045-3701

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