Fig. 4
Preparation and characterization of flexible nano-liposomes. A Scheme of flexible nano-liposomes’ preparation. B Particle size distribution and zeta potential of flexible nano-liposomes and flexible nano-liposomes@ r/si-ORF7. C TEM image of flexible nano-liposomes (left) and flexible nano-liposomes@ r/si-ORF7 (right) complexes marked by red arrow. Scale bar = 500 nm. D HaCaT cellular uptake of RNAFAM-delivered by flexible nano-liposomes after transfection at 0, 1, 5 h as observed under fluorescence microscope (Scale bar = 50 μm). RNAFAM was labled green and nuclei were labeled with Hoechst (blue). E The delivery efficiency of r/si-ORF7 with flexible nano-liposomes. The expression level of mature si-ORF7 was detected by RT-qPCR 48 h post-transfection