Fig. 3

R/si-ORF7 inhibited VZV replication in human ARPE-19 cells without toxicity. ARPE-19 cells were transfected with 10 nM of r/si-ORF7 6 h before infection by VZV v-Oka (A, B) or r-Oka (C–F) at moi of 0.3 and incubated for 2 days. A VZV gE expression was evaluated using an immunofluorescence or B western blotting. gE was stained green and nuclei were labeled with DAPI (blue). Scale bar = 100 μm. C GFP expression was observed using a fluorescence microscope at 1, 2, 3, and 4 dpi. Scale bar = 500 μm. D The fluorescence intensity in C. Values are the mean ± S.D. of triplicated treatments; **P < 0.01 relative to SSA. E Activity of luciferase was detected at 1, 2, 3, and 4 dpi. ***P < 0.001 relative to SSA.