Fig. 8

BNIP3 regulates the translocation of LC3 to mitochondria. A RAW 264.7 cells were treated with bafilomycin (100 nM) for 1 h and then infected with Mtb at an MOI of 1 for 48 h. Western blot analysis was performed using mitochondrial fractions from Mtb-infected macrophages. B RAW 264.7 cells were infected with Mtb for 48 h, and then stained with LC3 (red), LAMP1 (green), Tom20 (gray), and DAPI (blue). C Quantification data for LC3 and LAMP1 recruitment to mitochondria were collected from 100 cells from at five independent experiments. Quantification data in the B. D Ratio of LC3 and LAMP1 fluorescence intensity was observed in B (n = 5 per group). The data are presented as means ± SD of at least three independent experiments; *p < 0.05, **p < 0.01 ***p < 0.001