Fig. 6

Mitophagy receptors regulate Mtb. A RAW 264.7 cells were infected with Mtb (MOI = 1) for 24 and 48 h. B–G WT and BNIP3-KO RAW 264.7 cells were transfected with siRNA and then infected with Mtb (MOI = 1) for 48 h. B Mitophagy was measured in RAW 264.7 cells using the mitophagy detection kit (n = 4 per group). C MMP was measured in RAW 264.7 cells using JC-1 staining. Fluorescent intensity of JC-1 was detected at excitation wavelength 488 nm and emission wavelength 530 nm using flow cytometry (n = 4 per group). D WT and BNIP3-KO RAW 264.7 cells were incubated with MitoSOX Red to measure mROS levels. The mROS levels were analyzed using flow cytometry (n = 4 per group). E Intracellular survival was assayed by enumerating the CFU (n = 9 per group). F, G The analysis of proinflammatory cytokine levels was performed in macrophages at 48 h post infection (n = 6 per group). The data are presented as means ± SD of at least three independent experiments; *p < 0.05, **p < 0.01, ***p < 0.001